The engineered expression of secreted HSPB5-Fc in CHO cells exhibits cytoprotection in vitro.
BMC Biotechnol
; 21(1): 39, 2021 06 14.
Article
em En
| MEDLINE
| ID: mdl-34126963
ABSTRACT
BACKGROUND:
HSPB5 is an ATP-independent molecular chaperone that is induced by heat shock or other proteotoxic stresses. HSPB5 is cytoprotective against stress both intracellularly and extracellularly. It acts as a potential therapeutic candidate in ischemia-reperfusion and neurodegenerative diseases.RESULTS:
In this paper, we constructed a recombinant plasmid that expresses and extracellularly secrets a HSPB5-Fc fusion protein (sHSPB5-Fc) at 0.42 µg/ml in CHO-K1 cells. This sHSPB5-Fc protein contains a Fc-tag at the C-terminal extension of HSPB5, facilitating protein-affinity purification. Our study shows that sHSPB5-Fc inhibits heat-induced aggregation of citrate synthase in a time and dose dependent manner in vitro. Administration of sHSPB5-Fc protects lens epithelial cells against cisplatin- or UVB-induced cell apoptosis. It also decreases GFP-Httex1-Q74 insolubility, and reduces the size and cytotoxicity of GFP-Httex1-Q74 aggregates in PC-12 cells.CONCLUSION:
This recombinant sHSPB5-Fc exhibits chaperone activity to protect cells against proteotoxicity.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Substâncias Protetoras
/
Cadeia B de alfa-Cristalina
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article