Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes.
STAR Protoc
; 2(2): 100596, 2021 06 18.
Article
em En
| MEDLINE
| ID: mdl-34169290
ABSTRACT
During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca2+ activity can be performed continuously for 2-3 days and even up to several months. For complete details on the use and execution of this protocol, please refer to Gengatharan et al. (2021).
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Base de dados:
MEDLINE
Assunto principal:
Endoscópios
/
Células-Tronco Neurais
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article