Obtaining purified human intestinal epithelia for single-cell analysis and organoid culture.
STAR Protoc
; 2(2): 100597, 2021 06 18.
Article
em En
| MEDLINE
| ID: mdl-34169291
ABSTRACT
Here, we describe protocols for the preparation and dissociation of human fetal and pediatric intestinal tissue to a high-viability epithelial single-cell suspension. This epithelium-enriched single-cell suspension can then be used to generate single-cell RNA sequencing data as well as to create human intestinal organoids from both the fetal and pediatric intestine. Finally, this protocol details the dissociation of the intestinal organoids for use in single-cell analysis or passaging of organoids. For complete details on the use and execution of this protocol, please refer to Elmentaite et al. (2020).
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Organoides
/
Análise de Célula Única
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Mucosa Intestinal
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article