CD36 family members are TCR-independent ligands for CD1 antigen-presenting molecules.
Sci Immunol
; 6(60)2021 06 25.
Article
em En
| MEDLINE
| ID: mdl-34172588
ABSTRACT
CD1c presents lipid-based antigens to CD1c-restricted T cells, which are thought to be a major component of the human T cell pool. However, the study of CD1c-restricted T cells is hampered by the presence of an abundantly expressed, non-T cell receptor (TCR) ligand for CD1c on blood cells, confounding analysis of TCR-mediated CD1c tetramer staining. Here, we identified the CD36 family (CD36, SR-B1, and LIMP-2) as ligands for CD1c, CD1b, and CD1d proteins and showed that CD36 is the receptor responsible for non-TCR-mediated CD1c tetramer staining of blood cells. Moreover, CD36 blockade clarified tetramer-based identification of CD1c-restricted T cells and improved identification of CD1b- and CD1d-restricted T cells. We used this technique to characterize CD1c-restricted T cells ex vivo and showed diverse phenotypic features, TCR repertoire, and antigen-specific subsets. Accordingly, this work will enable further studies into the biology of CD1 and human CD1-restricted T cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas
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Subpopulações de Linfócitos T
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Apresentação de Antígeno
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Antígenos CD36
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Antígenos CD1
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article