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Genomic and Proteomic Study of Andreprevotia ripae Isolated from an Anthill Reveals an Extensive Repertoire of Chitinolytic Enzymes.
Lorentzen, Silje B; Arntzen, Magnus Ø; Hahn, Thomas; Tuveng, Tina R; Sørlie, Morten; Zibek, Susanne; Vaaje-Kolstad, Gustav; Eijsink, Vincent G H.
Afiliação
  • Lorentzen SB; Faculty of Chemistry, Biotechnology, and Food Science, NMBU - Norwegian University of Life Sciences, N-1433 Ås, Norway.
  • Arntzen MØ; Faculty of Chemistry, Biotechnology, and Food Science, NMBU - Norwegian University of Life Sciences, N-1433 Ås, Norway.
  • Hahn T; Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstraße 12, 70569 Stuttgart, Germany.
  • Tuveng TR; Faculty of Chemistry, Biotechnology, and Food Science, NMBU - Norwegian University of Life Sciences, N-1433 Ås, Norway.
  • Sørlie M; Faculty of Chemistry, Biotechnology, and Food Science, NMBU - Norwegian University of Life Sciences, N-1433 Ås, Norway.
  • Zibek S; Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstraße 12, 70569 Stuttgart, Germany.
  • Vaaje-Kolstad G; Faculty of Chemistry, Biotechnology, and Food Science, NMBU - Norwegian University of Life Sciences, N-1433 Ås, Norway.
  • Eijsink VGH; Faculty of Chemistry, Biotechnology, and Food Science, NMBU - Norwegian University of Life Sciences, N-1433 Ås, Norway.
J Proteome Res ; 20(8): 4041-4052, 2021 08 06.
Article em En | MEDLINE | ID: mdl-34191517
Chitin is an abundant natural polysaccharide that is hard to degrade because of its crystalline nature and because it is embedded in robust co-polymeric materials containing other polysaccharides, proteins, and minerals. Thus, it is of interest to study the enzymatic machineries of specialized microbes found in chitin-rich environments. We describe a genomic and proteomic analysis of Andreprevotia ripae, a chitinolytic Gram-negative bacterium isolated from an anthill. The genome of A. ripae encodes four secreted family GH19 chitinases of which two were detected and upregulated during growth on chitin. In addition, the genome encodes as many as 25 secreted GH18 chitinases, of which 17 were detected and 12 were upregulated during growth on chitin. Finally, the single lytic polysaccharide monooxygenase (LPMO) was strongly upregulated during growth on chitin. Whereas 66% of the 29 secreted chitinases contained two carbohydrate-binding modules (CBMs), this fraction was 93% (13 out of 14) for the upregulated chitinases, suggesting an important role for these CBMs. Next to an unprecedented multiplicity of upregulated chitinases, this study reveals several chitin-induced proteins that contain chitin-binding CBMs but lack a known catalytic function. These proteins are interesting targets for discovery of enzymes used by nature to convert chitin-rich biomass. The MS proteomic data have been deposited in the PRIDE database with accession number PXD025087.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Quitinases / Betaproteobacteria / Proteômica Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Quitinases / Betaproteobacteria / Proteômica Idioma: En Ano de publicação: 2021 Tipo de documento: Article