Structural characterization of two solute-binding proteins for N,N'-diacetylchitobiose/N,N',N''-triacetylchitotoriose of the gram-positive bacterium, Paenibacillus sp. str. FPU-7.

Itoh, Takafumi; Yaguchi, Misaki; Nakaichi, Akari; Yoda, Moe; Hibi, Takao; Kimoto, Hisashi

Structural characterization of two solute-binding proteins for N,N'-diacetylchitobiose/N,N',N''-triacetylchitotoriose of the gram-positive bacterium, Paenibacillus sp. str. FPU-7.

Itoh, Takafumi; Yaguchi, Misaki; Nakaichi, Akari; Yoda, Moe; Hibi, Takao; Kimoto, Hisashi.

Afiliação

Itoh T; Department of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
Yaguchi M; Department of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
Nakaichi A; Department of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
Yoda M; Department of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
Hibi T; Department of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
Kimoto H; Department of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Matsuokakenjyoujima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.

J Struct Biol X ; 5: 100049, 2021.

Article em En | MEDLINE | ID: mdl-34195603

Palavras-chave

ABC transporter; ABC, ATP-binding cassette; Chitin oligosaccharide; DSF, differential scanning fluorimetry; GH, glycoside hydrolase; GlcN, D-glucosamine; GlcNAc, N-acetyl-D-glucosamine; OD600, optical density at 600 nm; PDB, Protein Data Bank; PTS, phosphoenolpyruvate phosphotransferase system; Paenibacillus; RU, response unit; SBP, solute binding protein; Se-Met, selenomethionine; Solute binding protein; TMD, transmembrane domain; Two-component regulatory system; a.a., amino acid; r.m.s.d., root mean-square deviation

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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article