Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq.
Nat Biotechnol
; 39(12): 1517-1520, 2021 12.
Article
em En
| MEDLINE
| ID: mdl-34211161
ABSTRACT
Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized using dimeric nucleotide building blocks that allow error detection. We illustrate the use of the method for evaluating barcode assignment accuracy, differential isoform usage in myeloma cell lines, and fusion transcript detection in a sarcoma cell line.
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Base de dados:
MEDLINE
Assunto principal:
Sequenciamento por Nanoporos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article