Spleen route accelerates engraftment of human hematopoietic stem cells.
Biochem Biophys Res Commun
; 569: 23-28, 2021 09 10.
Article
em En
| MEDLINE
| ID: mdl-34216994
ABSTRACT
Intravenous injections of human hematopoietic stem cells (hHSCs) is routinely used in clinic and for modeling hematopoiesis in mice. However, unspecific dilution in vascular system and non-hematopoietic organs challenges engraftment efficiency. Although spleen is capable of extra medullar hematopoiesis, its ability to support human HSC transplantation has never been evaluated. We demonstrate that intra-splenic injection results in high and sustained engraftment of hHSCs into immune-deficient mice, with higher chimerisms than with intravenous or intra-femoral injections. Our results support that spleen microenvironment provides a niche for HSCs amplification and offers a new route for efficient HSC transplantation.
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Base de dados:
MEDLINE
Assunto principal:
Baço
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Células-Tronco Hematopoéticas
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Transplante de Células-Tronco Hematopoéticas
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Sobrevivência de Enxerto
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article