Get ready for the CRISPR/Cas system: A beginner's guide to the engineering and design of guide RNAs.
J Gene Med
; 23(11): e3377, 2021 11.
Article
em En
| MEDLINE
| ID: mdl-34270141
ABSTRACT
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a state-of-the-art tool for versatile genome editing that has advanced basic research dramatically, with great potential for clinic applications. The system consists of two key molecules a CRISPR-associated (Cas) effector nuclease and a single guide RNA. The simplicity of the system has enabled the development of a wide spectrum of derivative methods. Almost any laboratory can utilize these methods, although new users may initially be confused when faced with the potentially overwhelming abundance of choices. Cas nucleases and their engineering have been systematically reviewed previously. In the present review, we discuss single guide RNA engineering and design strategies that facilitate more efficient, more specific and safer gene editing.
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Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
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RNA Guia de Cinetoplastídeos
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Sistemas CRISPR-Cas
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Edição de Genes
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article