Rapid protein delivery to living cells for biomolecular investigation.
Biochem Biophys Res Commun
; 570: 82-88, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34274850
ABSTRACT
The lack of a simple, fast and efficient method for protein delivery is limiting the widespread application of in-cell experiments, which are crucial for understanding the cellular function. We present here an innovative strategy to deliver proteins into both prokaryotic and eukaryotic cells, exploiting thermal vesiculation. This method allows to internalize substantial amounts of proteins, with different molecular weight and conformation, without compromising the structural properties and cell viability. Characterizing proteins in a physiological environment is essential as the environment can dramatically affect the conformation and dynamics of biomolecules as shown by in-cell EPR spectra vs those acquired in buffer solution. Considering its versatility, this method opens the possibility to scientists to study proteins directly in living cells through a wide range of techniques.
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Base de dados:
MEDLINE
Assunto principal:
Bioquímica
/
Proteínas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article