A method for constructing GLP-1 overexpression intestinal organoids.
Yi Chuan
; 43(7): 694-703, 2021 Jul 20.
Article
em En
| MEDLINE
| ID: mdl-34284984
ABSTRACT
As a potent insulinotrophic hormone, glucagon-like peptide 1 (GLP-1) is mainly secreted by intestinal L cells, which can effectively promote the release of insulin and thus reduce blood glucose. Therefore, GLP-1 and its analogs have a good prospect in the treatment of type 2 diabetes. In this study, we constructed mouse intestinal organoids that overexpress GLP-1 by optimizing the GLP-1 lentivirus infection method. We found that supernatants secreted by the GLP-1 overexpression organoids effectively enhanced glucose tolerance in wild-type and diabetic mouse. Thus, the GLP-1 overexpression organoids built in this study may provide a novel strategy for the treatment of type 2 diabetes.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Diabetes Mellitus Tipo 2
/
Peptídeo 1 Semelhante ao Glucagon
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article