A 'smart' aptamer-functionalized continuous label-free cell catch-transport-release system.
J Mater Chem B
; 9(35): 7196-7204, 2021 09 15.
Article
em En
| MEDLINE
| ID: mdl-34291267
ABSTRACT
Label-free cell sorting devices are of great significance for biomedical research and clinical therapeutics. However, current platforms for label-free cell sorting cannot achieve continuity and selectivity simultaneously, resulting in complex steps and limited reliability. Here, an immunoaffinity-based cell catch-transport-release thermo-chemo-mechanical coupling hydrogel (iCatch) device is reported. It contains a temperature-responsive hydrogel that can generate spatial movement synergically with the reversible binding of affinity handle modified. The functionalized hydrogel is embedded inside a biphasic microfluidic platform to enable cell transportation between the flows. The cell sorting capability and biocompatibility of the iCatch device were validated with CCRF-CEM cells as a proof-of-concept, and CCRF-CEM-specific aptamers with thermo-responsive affinity as well as a hydrogel with temperature-dependent volume were employed accordingly. A cell catching efficiency of â¼40% and a recovery rate of â¼70% were achieved. The iCatch device provides a high-throughput (â¼900 cells mm-1 s-1) platform for cell sorting and is ultimately valuable for downstream biomedical applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
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Separação Celular
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Hidrogéis
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Aptâmeros de Nucleotídeos
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Dispositivos Lab-On-A-Chip
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article