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Exploring the Tunability and Dynamic Properties of MarR-PmarO Sensor System in Escherichia coli.
Zou, Yusong; Li, Chenyi; Zhang, Ruihua; Jiang, Tian; Liu, Ning; Wang, Jian; Wang, Xianqiao; Yan, Yajun.
Afiliação
  • Zou Y; School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
  • Li C; School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
  • Zhang R; School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
  • Jiang T; School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
  • Liu N; School of Environmental, Civil, Agricultural, and Mechanical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
  • Wang J; School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
  • Wang X; School of Environmental, Civil, Agricultural, and Mechanical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
  • Yan Y; School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.
ACS Synth Biol ; 10(8): 2076-2086, 2021 08 20.
Article em En | MEDLINE | ID: mdl-34319697
ABSTRACT
Transcriptional factor-based biosensors (TFBs) have been widely used in dynamic pathway control or high-throughput screening. Here, we systematically explored the tunability of a salicylic acid responsive regulator MarR from Escherichia coli aiming to explore its engineering potential. The effect of endogenous MarR in E. coli on the MarR-PmarO biosensor system was investigated. Furthermore, to investigate the function of marO binding boxes in this biosensor system, a series of hybrid promoters were constructed by placing the marO binding boxes in the strong constitutive pL promoter. The engineered hybrid promoters became responsive to MarR and salicylic acid. To further study the influence of each nucleotide in the marO box on MarR binding, we employed dynamic modeling to simulate the interaction and binding energy between each nucleotide in the marO boxes with the corresponding residues on MarR. Guided by the results of the simulation, we introduced mutations to key positions on the hybrid promoters and investigated corresponding dynamic performance. Two promoter variants I12AII4T and I12AII14T that exhibited improved responsive strengths and shifted dynamic ranges were obtained, which can be beneficial for future metabolic engineering research.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Técnicas Biossensoriais / Ácido Salicílico / Elementos de Resposta / Proteínas de Escherichia coli / Escherichia coli / Modelos Biológicos Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Técnicas Biossensoriais / Ácido Salicílico / Elementos de Resposta / Proteínas de Escherichia coli / Escherichia coli / Modelos Biológicos Idioma: En Ano de publicação: 2021 Tipo de documento: Article