Multiplexed Tissue Tomography.
Methods Mol Biol
; 2350: 77-93, 2021.
Article
em En
| MEDLINE
| ID: mdl-34331280
ABSTRACT
Multiplexed tissue tomography enables comprehensive spatial analysis of markers within a whole tissue or thick tissue section. Clearing agents are often used to make tissue transparent and facilitate deep tissue imaging. Many methods of clearing and tissue tomography are currently used in a variety of tissue types. Here we detail a workflow known as transparent tissue tomography (T3), which builds upon previous methods and can be applied to difficult to clear tissues such as tumors.
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Base de dados:
MEDLINE
Assunto principal:
Tomografia
/
Imunofluorescência
/
Imagem Óptica
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Histocitoquímica
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article