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Lnc-STYK1-2 regulates bladder cancer cell proliferation, migration, and invasion by targeting miR-146b-5p expression and AKT/STAT3/NF-kB signaling.
Dai, Ranran; Jiang, Qingping; Zhou, You; Lin, Ruifeng; Lin, Hai; Zhang, Yumin; Zhang, Jinhu; Gao, Xingcheng.
Afiliação
  • Dai R; Guangdong Key Laboratory of Urology, Guangzhou Medical University, Guangzhou, China.
  • Jiang Q; Department of Pathology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
  • Zhou Y; Guangdong Key Laboratory of Urology, Guangzhou Medical University, Guangzhou, China.
  • Lin R; Guangdong Key Laboratory of Urology, Guangzhou Medical University, Guangzhou, China.
  • Lin H; Guangdong Key Laboratory of Urology, Guangzhou Medical University, Guangzhou, China.
  • Zhang Y; Department of Children's Stomatology, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China.
  • Zhang J; Guangdong Key Laboratory of Urology, Guangzhou Medical University, Guangzhou, China.
  • Gao X; Guangdong Key Laboratory of Urology, Guangzhou Medical University, Guangzhou, China. xchgao@gzhmu.edu.cn.
Cancer Cell Int ; 21(1): 408, 2021 Jul 31.
Article em En | MEDLINE | ID: mdl-34332611
ABSTRACT

BACKGROUND:

Epigenetic modulation by noncoding RNAs substantially contributes to human cancer development, but noncoding RNAs involvement in bladder cancer remains poorly understood. This study investigated the role of long noncoding RNA (lncRNA) lnc-STYK1-2 in tumorigenesis in cancerous bladder cells.

METHODS:

Differential lncRNA and mRNA profiles were characterized by high-throughput RNA sequencing combined with validation via quantitative PCR. Bladder cancer cell proliferation was assessed through MTS, and bladder cancer cell migration and invasion were assessed through a Transwell system. The in vivo tumorigenesis of bladder cancer cells was evaluated using the cancer cell line-based xenograft model. The dual-luciferase reporter assay verified the association of miR-146b-5p with lnc-STYK1-2 and the target gene. Protein abundances and phosphorylation were detected by Western blotting.

RESULTS:

Alterations in lncRNA profiles, including decreased lnc-STYK1-2 expression, were detected in bladder cancer tissues compared with adjacent noncancerous tissues. lnc-STYK1-2 silencing effectively promoted proliferation, migration, and invasion in two bladder cancer cell lines, 5637 and T24, and their tumorigenesis in nude mice. lnc-STYK1-2 siRNA promoted miR-146b-5p and reduced ITGA2 expression in bladder cancer cells. Moreover, miR-146b-5p suppressed ITGA2 expression in bladder cancer cells through direct association. Also, lnc-STYK1-2 directly associated with miR-146b-5p. Finally, miR-146b-5p inhibitors abrogated the alterations in bladder cell functions, ITGA2 expression, and phosphorylation of AKT, STAT3, and P65 proteins in 5637 and T24 cells induced by lnc-STYK1-2 silencing.

CONCLUSION:

lnc-STYK1-2 inhibited bladder cancer cell proliferation, migration, and tumorigenesis by targeting miR-146b-5p to regulate ITGA2 expression and AKT/STAT3/NF-kB signaling.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article