Single-molecule fluorescence vistas of how lipids regulate membrane proteins.
Biochem Soc Trans
; 49(4): 1685-1694, 2021 08 27.
Article
em En
| MEDLINE
| ID: mdl-34346484
ABSTRACT
The study of membrane proteins is undergoing a golden era, and we are gaining unprecedented knowledge on how this key group of proteins works. However, we still have only a basic understanding of how the chemical composition and the physical properties of lipid bilayers control the activity of membrane proteins. Single-molecule (SM) fluorescence methods can resolve sample heterogeneity, allowing to discriminate between the different molecular populations that biological systems often adopt. This short review highlights relevant examples of how SM fluorescence methodologies can illuminate the different ways in which lipids regulate the activity of membrane proteins. These studies are not limited to lipid molecules acting as ligands, but also consider how the physical properties of the bilayer can be determining factors on how membrane proteins function.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metabolismo dos Lipídeos
/
Imagem Individual de Molécula
/
Bicamadas Lipídicas
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article