In vivo endocannabinoid dynamics at the timescale of physiological and pathological neural activity.
Neuron
; 109(15): 2398-2403.e4, 2021 08 04.
Article
em En
| MEDLINE
| ID: mdl-34352214
ABSTRACT
The brain's endocannabinoid system is a powerful controller of neurotransmitter release, shaping synaptic communication under physiological and pathological conditions. However, our understanding of endocannabinoid signaling in vivo is limited by the inability to measure their changes at timescales commensurate with the high lability of lipid signals, leaving fundamental questions of whether, how, and which endocannabinoids fluctuate with neural activity unresolved. Using novel imaging approaches in awake behaving mice, we now demonstrate that the endocannabinoid 2-arachidonoylglycerol, not anandamide, is dynamically coupled to hippocampal neural activity with high spatiotemporal specificity. Furthermore, we show that seizures amplify the physiological endocannabinoid increase by orders of magnitude and drive the downstream synthesis of vasoactive prostaglandins that culminate in a prolonged stroke-like event. These results shed new light on normal and pathological endocannabinoid signaling in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Convulsões
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Transmissão Sináptica
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Endocanabinoides
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Região CA1 Hipocampal
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article