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Parasporin A13-2 of Bacillus thuringiensis Isolates from the Papaloapan Region (Mexico) Induce a Cytotoxic Effect by Late Apoptosis against Breast Cancer Cells.
Borin, Diego Becker; Castrejón-Arroyo, Karen; Cruz-Nolasco, Alain; Peña-Rico, Miguel; Sagrillo, Michele Rorato; Santos, Roberto C V; Baco, Lucas Silva de; Pérez-Picaso, Lemuel; Camacho, Luz; Navarro-Mtz, A Karin.
Afiliação
  • Borin DB; Instituto de Biotecnología, Universidad del Papaloapan, Tuxtepec, Oaxaca 68301, Mexico.
  • Castrejón-Arroyo K; División de Estudios de Posgrado, Universidad del Papaloapan, Tuxtepec, Oaxaca 68301, Mexico.
  • Cruz-Nolasco A; División de Estudios de Posgrado, Universidad del Papaloapan, Tuxtepec, Oaxaca 68301, Mexico.
  • Peña-Rico M; Instituto de Biotecnología, Universidad del Papaloapan, Tuxtepec, Oaxaca 68301, Mexico.
  • Sagrillo MR; Graduate Program in Nanoscience, Franciscan University, Santa Maria, Rio Grande do Sul 97010-032, Brazil.
  • Santos RCV; Oral Microbiology Laboratory, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul 97065-060, Brazil.
  • Baco LS; Oncologia, Franciscan University, Santa Maria, Rio Grande do Sul 97015-450, Brazil.
  • Pérez-Picaso L; Instituto de Química Aplicada, Universidad del Papaloapan, Tuxtepec, Oaxaca 68301, Mexico.
  • Camacho L; Laboratorio de Nutrición Experimental, Instituto Nacional de Pediatría, Ciudad de México 4530, Mexico.
  • Navarro-Mtz AK; Instituto de Biotecnología, Universidad del Papaloapan, Tuxtepec, Oaxaca 68301, Mexico.
Toxins (Basel) ; 13(7)2021 07 09.
Article em En | MEDLINE | ID: mdl-34357948
The protein A13-2 was obtained from Bacillus thuringiensis strains isolated from the Papaloapan watershed region (Oaxaca, Mexico). The cytotoxic activity of parasporal inclusions was studied against breast cancer cell line (MCF-7) and normal cell (human peripheral blood mononuclear cells). The MTT, the formation of reactive species, nitric oxide, free cell DNA, and the type of death cellular were assessed. The protein A13-2 shows the highest cytotoxic activity against MCF-7 (13% cell viability at 6 µg/mL), the extracellular DNA increases, and it shows no stress for reactive species or nitric oxide. Besides, the A13-2 parasporin shows no toxicity to peripheral blood mononuclear cells, and it does not generate changes in nitric oxide levels or free cell DNA. Due to that, the cytotoxic effect of A13-2 was specific for MCF-7, and it does not affect normal cells. According to microscopy and flow cytometry, A13-2 parasporin leads to the death of MCF-7 cells by late apoptosis together with necrosis and without allowing the triggering of the survival mechanisms. When analyzed together, our results show for the first time that the A13-2 protein isolated from Mexican strains of B. thuringiensis preferentially kills MCF- 7 (cancer cells) over HEK 293 and PBMC cell lines (normal cells), thus representing a promising alternative for the treatment of cancer breast.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Endotoxinas / Antineoplásicos Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Endotoxinas / Antineoplásicos Idioma: En Ano de publicação: 2021 Tipo de documento: Article