Low Input Targeted Chromatin Capture (Low-T2C).

Boltsis, Ilias; Nowosad, Karol; Brouwer, Rutger W W; Tylzanowski, Przemko; van IJcken, Wilfred F J; Huylebroeck, Danny; Grosveld, Frank; Kolovos, Petros

Boltsis, Ilias; Nowosad, Karol; Brouwer, Rutger W W; Tylzanowski, Przemko; van IJcken, Wilfred F J; Huylebroeck, Danny; Grosveld, Frank; Kolovos, Petros.

Afiliação

Boltsis I; Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Nowosad K; Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Brouwer RWW; Department of Biochemistry and Molecular Biology, Medical University of Lublin, Lublin, Poland.
Tylzanowski P; The Postgraduate School of Molecular Medicine, Medical University of Warsaw, Warsaw, Poland.
van IJcken WFJ; Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Huylebroeck D; Center for Biomics, Erasmus Medical Center, Rotterdam, The Netherlands.
Grosveld F; Department of Biochemistry and Molecular Biology, Medical University of Lublin, Lublin, Poland.
Kolovos P; Department of Development and Regeneration, Skeletal Biology and Engineering Research Center, University of Leuven, Leuven, Belgium.

Methods Mol Biol ; 2351: 165-179, 2021.

Article em En | MEDLINE | ID: mdl-34382189

RESUMO

Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.

Assuntos

Montagem e Desmontagem da Cromatina; Cromatina/genética; Biologia Computacional/métodos; Cromatina/química; Cromatina/metabolismo; Mapeamento Cromossômico; Regulação da Expressão Gênica; Biblioteca Gênica; Genômica/métodos; Reprodutibilidade dos Testes

Palavras-chave

C-technologies; Chromatin; Chromatin conformation capture; Genome architecture; T2C; Three-dimensional (3D) organization; Topologically associating domains (TADs)

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cromatina / Biologia Computacional / Montagem e Desmontagem da Cromatina Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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