Diazinon toxicity in hepatic and spleen mononuclear cells is associated to early induction of oxidative stress.
Int J Environ Health Res
; 32(10): 2309-2323, 2022 Oct.
Article
em En
| MEDLINE
| ID: mdl-34404283
ABSTRACT
Diazinon is an organophosphorus pesticide, which may have potential toxic effects on the liver and immune system; however, the underlying mechanisms remain mostly unidentified. This work is aimed at evaluating the oxidative stress and cell cycle alterations elicited by low-dose diazinon in a rat liver cell line (BRL-3A) and spleen mononuclear cells (SMC) from Wistar rats. Diazinon (10-50 µM) caused early reactive oxygen species (ROS) generation (from 4 h) as well as increased O2â¢- level (from 0.5 h), which led to subsequent lipid peroxidation at 24 h, in BRL-3A cells. In SMC, diazinon (20 µM) produced similar increases in ROS levels, at 4 and 24 h, with the highest O2â¢- level being found at 4 h. Low-dose diazinon induced G1-phase arrest and cell death in hepatic cells and SMC. Therefore, diazinon could affect the liver and the immunological system through the premature oxidative stress induction.Abbreviations O2â¢- superoxide anion radical; ROS reactive oxygen species; SMC spleen mononuclear cells; TBARS thiobarbituric acid reactive substances.
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Base de dados:
MEDLINE
Assunto principal:
Praguicidas
/
Diazinon
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article