A human forebrain organoid model of fragile X syndrome exhibits altered neurogenesis and highlights new treatment strategies.
Nat Neurosci
; 24(10): 1377-1391, 2021 10.
Article
em En
| MEDLINE
| ID: mdl-34413513
ABSTRACT
Fragile X syndrome (FXS) is caused by the loss of fragile X mental retardation protein (FMRP), an RNA-binding protein that can regulate the translation of specific mRNAs. In this study, we developed an FXS human forebrain organoid model and observed that the loss of FMRP led to dysregulated neurogenesis, neuronal maturation and neuronal excitability. Bulk and single-cell gene expression analyses of FXS forebrain organoids revealed that the loss of FMRP altered gene expression in a cell-type-specific manner. The developmental deficits in FXS forebrain organoids could be rescued by inhibiting the phosphoinositide 3-kinase pathway but not the metabotropic glutamate pathway disrupted in the FXS mouse model. We identified a large number of human-specific mRNAs bound by FMRP. One of these human-specific FMRP targets, CHD2, contributed to the altered gene expression in FXS organoids. Collectively, our study revealed molecular, cellular and electrophysiological abnormalities associated with the loss of FMRP during human brain development.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Prosencéfalo
/
Neurogênese
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Síndrome do Cromossomo X Frágil
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article