1H, 15N, and 13C resonance assignments of the SH3-like tandem domain of human KIN protein.
Biomol NMR Assign
; 15(2): 449-453, 2021 10.
Article
em En
| MEDLINE
| ID: mdl-34417717
ABSTRACT
KIN is a DNA/RNA-binding protein conserved evolutionarily from yeast to humans and expressed ubiquitously in mammals. It is an essential nuclear protein involved in numerous cellular processes, such as DNA replication, class-switch recombination, cell cycle regulation, and response to UV or ionizing radiation-induced DNA damage. The C-terminal region of the human KIN (hKIN) protein is composed of an SH3-like tandem domain, which is crucial for the anti-proliferation effect of the full-length protein. Herein, we present the 1H, 15N, and 13C resonances assignment of the backbone and side chains for the SH3-like tandem domain of the hKIN protein, as well as the secondary structure prediction based on the assigned chemical shifts using TALOS-N software. This work prepares the ground for future studies of RNA-binding and backbone dynamics.
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MEDLINE
Assunto principal:
Domínios de Homologia de src
Idioma:
En
Ano de publicação:
2021
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Article