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Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D3 on assay performance.
Wise, Stephen A; Camara, Johanna E; Burdette, Carolyn Q; Hahm, Grace; Nalin, Federica; Kuszak, Adam J; Merkel, Joyce; Durazo-Arvizu, Ramón A; Williams, Emma L; Hoofnagle, Andrew N; Ivison, Fiona; Fischer, Ralf; van den Ouweland, Jody M W; Ho, Chung S; Law, Emmett W K; Simard, Jean-Nicolas; Gonthier, Renaud; Holmquist, Brett; Meadows, Sarah; Cox, Lorna; Robyak, Kimberly; Creer, Michael H; Fitzgerald, Robert; Clarke, Michael W; Breen, Norma; Lukas, Pierre; Cavalier, Étienne; Sempos, Christopher T.
Afiliação
  • Wise SA; Office of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD, 20892, USA. stephen.wise@nih.gov.
  • Camara JE; Scientist Emeritus, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA. stephen.wise@nih.gov.
  • Burdette CQ; Chemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA.
  • Hahm G; Chemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA.
  • Nalin F; Chemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA.
  • Kuszak AJ; Chemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA.
  • Merkel J; Office of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
  • Durazo-Arvizu RA; Office of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
  • Williams EL; Biostatistics Core, The Sabin Research Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90027, USA.
  • Hoofnagle AN; Imperial Healthcare NHS Trust, London, W6 8RF, UK.
  • Ivison F; Department of Laboratory Medicine, University of Washington, Seattle, WA, 98185, USA.
  • Fischer R; Central Manchester Foundation Trust, Manchester, M13 9WL, UK.
  • van den Ouweland JMW; Chromsystems Instruments & Chemicals GmbH, 82166, Gräfelfing, Germany.
  • Ho CS; Canisius Wilhelmina Hospital (CWZ), 6532, SZ, Nijmegen, The Netherlands.
  • Law EWK; Biochemical Mass Spectrometry Unit, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, Hong Kong.
  • Simard JN; Biochemical Mass Spectrometry Unit, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, Hong Kong.
  • Gonthier R; Endoceutics Inc., Québec, G1P 4P5, Canada.
  • Holmquist B; Endoceutics Inc., Québec, G1P 4P5, Canada.
  • Meadows S; Endocrine Sciences, LabCorp Specialty Testing Group, Agoura Hills, CA, 91301, USA.
  • Cox L; Medical Research Council (MRC) Elsie Widdowson Laboratory (closed Dec. 2018), Cambridge, UK.
  • Robyak K; NIHR BRC Nutritional Biomarker Laboratory, MRC Epidemiology Unit, University of Cambridge, Cambridge, CB2 0SL, UK.
  • Creer MH; Medical Research Council (MRC) Elsie Widdowson Laboratory (closed Dec. 2018), Cambridge, UK.
  • Fitzgerald R; NIHR BRC Nutritional Biomarker Laboratory, MRC Epidemiology Unit, University of Cambridge, Cambridge, CB2 0SL, UK.
  • Clarke MW; College of Medicine, Penn State University, Hershey, PA, 17033, USA.
  • Breen N; College of Medicine, Penn State University, Hershey, PA, 17033, USA.
  • Lukas P; Health Clinical Laboratories, University of California at San Diego, La Jolla, CA, 92093, USA.
  • Cavalier É; Metabolomics Australia, Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA, 6009, Australia.
  • Sempos CT; Waters Technologies Ireland Ltd., Wexford, Y35 D431, Ireland.
Anal Bioanal Chem ; 414(1): 333-349, 2022 Jan.
Article em En | MEDLINE | ID: mdl-34432104
ABSTRACT
An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays used for the determination of serum total 25-hydroxyvitamin D (25(OH)D), which is the sum of 25-hydroxyvitamin D2 (25(OH)D2) and 25-hydroxyvitamin D3 (25(OH)D3). A set of 50 single-donor samples was assigned target values for concentrations of 25(OH)D2, 25(OH)D3, 3-epi-25-hydroxyvitamin D3 (3-epi-25(OH)D3), and 24R,25-dihydroxyvitamin D3 (24R,25(OH)2D3) using isotope dilution liquid chromatography - tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 1 includes results from 14 laboratories using 14 custom LC-MS/MS assays. Assay performance was evaluated using mean % bias compared to the assigned target values and using linear regression analysis of the test assay mean results and the target values. Only 53% of the LC-MS/MS assays met the VDSP criterion of mean % bias ≤ |±5%|. For the LC-MS/MS assays not meeting the ≤ |±5%| criterion, four assays had mean % bias of between 12 and 21%. Based on multivariable regression analysis using the concentrations of the four individual vitamin D metabolites in the 50 single-donor samples, the performance of several LC-MS/MS assays was found to be influenced by the presence of 3-epi-25(OH)D3. The results of this interlaboratory study represent the most comprehensive comparison of LC-MS/MS assay performance for serum total 25(OH)D and document the significant impact of the lack of separation of 3-epi-25(OH)D3 and 25(OH)D3 on assay performance, particularly with regard to mean % bias.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vitamina D / Espectrometria de Massas em Tandem Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vitamina D / Espectrometria de Massas em Tandem Idioma: En Ano de publicação: 2022 Tipo de documento: Article