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Analysis of the ternary antiretroviral therapy dolutegravir, lamivudine and abacavir using UV spectrophotometry and chemometric tools.
Serag, Ahmed; Hasan, Mohamed A; Tolba, Enas H; Abdelzaher, Ahmed M; Elmaaty, Ayman Abo.
Afiliação
  • Serag A; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11751, Egypt. Electronic address: Ahmedserag777@azhar.edu.eg.
  • Hasan MA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11751, Egypt.
  • Tolba EH; National Organization for Drug Control and Research (NODCAR), Giza, P.O. Box 35521, Egypt.
  • Abdelzaher AM; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11751, Egypt.
  • Elmaaty AA; Department of Medicinal Chemistry, Faculty of Pharmacy, Port Said University, Port Said 42526, Egypt. Electronic address: ayman.mohamed@pharm.psu.edu.eg.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120334, 2022 Jan 05.
Article em En | MEDLINE | ID: mdl-34481252
ABSTRACT
Herein, a simple spectrophotometric method coupled with chemometric techniques i.e. partial least square (PLS) and genetic algorithm (GA) were utilized for the simultaneous determination of the vital ternary antiretroviral therapy dolutegravir (DTG), lamivudine (LMV), and abacavir (ACV) in their combined dosage form. Calibration (25 samples) and validation (13 samples) sets were prepared for these drugs at different concentrations via implementing partial factorial experimental designs. The zero order UV spectra of calibration and validation sets were measured and then subjected for further chemometric analysis. Partial least squares with/without variable selection procedures i.e. genetic algorithm (GA) were utilized to untangle the UV spectral overlapping of these mixtures. Cross-validation and external validation methods were applied to compare the performance of these chemometric techniques in terms of accuracy and predictive abilities. It was found that six latent variables were optimum for modelling DTG, four latent variables for modelling LMV and three latent variables for modelling ACV. Although, good recoveries with prompt predictive ability were attained by these PLS, GA-PLS showed better analytical performance owing to its capability to remove redundant variables i.e. the number of absorbance variables have been reduced to about 21-29%. The proposed chemometric methods can be reliably applied for simultaneous determination of DTG, LMV, and ACV in their laboratory prepared mixtures and pharmaceutical preparation posing these chemometric methods as worthy and substantial analytical tools in in-process testing and quality control analysis of many antiretroviral pharmaceutical preparations.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por HIV / Lamivudina Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por HIV / Lamivudina Idioma: En Ano de publicação: 2022 Tipo de documento: Article