Improving the alkaline stability of pepsin through rational protein design using renin, an alkaline-stable aspartic protease, as a structural and functional reference.

Grahame, Douglas A S; Dupuis, John H; Bryksa, Brian C; Tanaka, Takuji; Yada, Rickey Y

Grahame, Douglas A S; Dupuis, John H; Bryksa, Brian C; Tanaka, Takuji; Yada, Rickey Y.

Afiliação

Grahame DAS; Department of Food Science, Ontario Agricultural College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Dupuis JH; Food, Nutrition, and Health Program, Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
Bryksa BC; Department of Food Science, Ontario Agricultural College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Tanaka T; Department of Food and Bioproduct Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.
Yada RY; Food, Nutrition, and Health Program, Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. Electronic address: rickey.yada@ubc.ca.

Enzyme Microb Technol ; 150: 109871, 2021 Oct.

Article em En | MEDLINE | ID: mdl-34489030

Assuntos

Pepsina A; Renina; Sequência de Aminoácidos; Ácido Aspártico Endopeptidases/metabolismo; Ligação de Hidrogênio; Pepsina A/metabolismo

Palavras-chave

Aspartic protease; Pepsin; Rational re-design; Renin; Structure-Function; pH stability

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pepsina A / Renina Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pepsina A / Renina Idioma: En Ano de publicação: 2021 Tipo de documento: Article