Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants.
Proc Natl Acad Sci U S A
; 118(37)2021 09 14.
Article
em En
| MEDLINE
| ID: mdl-34497125
ABSTRACT
Absolute quantification measurements (copies per cell) of peptide major histocompatibility complex (pMHC) antigens are necessary to inform targeted immunotherapy drug design; however, existing methods for absolute quantification have critical limitations. Here, we present a platform termed SureQuant-IsoMHC, utilizing a series of pMHC isotopologues and internal standard-triggered targeted mass spectrometry to generate an embedded multipoint calibration curve to determine endogenous pMHC concentrations for a panel of 18 tumor antigens. We apply SureQuant-IsoMHC to measure changes in expression of our target panel in a melanoma cell line treated with a MEK inhibitor and translate this approach to estimate antigen concentrations in melanoma tumor biopsies.
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Base de dados:
MEDLINE
Assunto principal:
Benzimidazóis
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Antígenos de Histocompatibilidade Classe I
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Apresentação de Antígeno
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MAP Quinase Quinase 1
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Melanoma
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Antígenos de Neoplasias
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article