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Beyond survival experiments: using biomarkers of oxidative stress and neurotoxicity to assess vulnerability of subterranean fauna to climate change.
Pallarés, Susana; Sanchez-Hernandez, Juan C; Colado, Raquel; Balart-García, Pau; Comas, Jordi; Sánchez-Fernández, David.
Afiliação
  • Pallarés S; Marine Biology and Ecology Research Centre, School of Biological and Marine Sciences, University of Plymouth, Drake Circus, Plymouth, PL4 8AA, UK.
  • Sanchez-Hernandez JC; Departamento de Ciencias Ambientales, Instituto de Ciencias Ambientales, Universidad de Castilla-La Mancha, Avenida Carlos III, Toledo, 45071, Spain.
  • Colado R; Departamento de Ciencias Ambientales, Instituto de Ciencias Ambientales, Universidad de Castilla-La Mancha, Avenida Carlos III, Toledo, 45071, Spain.
  • Balart-García P; Departamento de Ciencias Ambientales, Instituto de Ciencias Ambientales, Universidad de Castilla-La Mancha, Avenida Carlos III, Toledo, 45071, Spain.
  • Comas J; Departamento de Ecología e Hidrología, Universidad de Murcia, Campus de Espinardo, Murcia, 30100, Spain.
  • Sánchez-Fernández D; Water and cave beetle evolution Lab, Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Maritim de la Barceloneta 37 Barcelona, 08003, Spain.
Conserv Physiol ; 8(1): coaa067, 2020.
Article em En | MEDLINE | ID: mdl-34504711
Accurate assessments of species vulnerability to climate change need to consider the physiological capacity of organisms to deal with temperature changes and identify early signs of thermally induced stress. Oxidative stress biomarkers and acetylcholinesterase activity are useful proxies of stress at the cellular and nervous system level. Such responses are especially relevant for poor dispersal organisms with limited capacity for behavioural thermoregulation, like deep subterranean species. We combined experimental measurements of upper lethal thermal limits, acclimation capacity and biomarkers of oxidative stress and neurotoxicity to assess the impact of heat stress (20°C) at different exposure times (2 and 7 days) on the Iberian endemic subterranean beetle Parvospeonomus canyellesi. Survival response (7 days of exposure) was similar to that reported for other subterranean specialist beetles (high survival up to 20°C but no above 23°C). However, a low physiological plasticity (i.e. incapacity to increase heat tolerance via acclimation) and signs of impairment at the cellular and nervous system level were observed after 7 days of exposure at 20°C. Such sublethal effects were identified by significant differences in total antioxidant capacity, glutathione S-transferase activity, the ratio of reduced to oxidized forms of glutathione and acetylcholinesterase activity between the control (cave temperature) and 20°C treatment. At 2 days of exposure, most biomarker values indicated some degree of oxidative stress in both the control and high-temperature treatment, likely reflecting an initial altered physiological status associated to factors other than temperature. Considering these integrated responses and the predicted increase in temperature in its unique locality, P. canyellesi would have a narrower thermal safety margin to face climate change than that obtained considering only survival experiments. Our results highlight the importance of exploring thermally sensitive processes at different levels of biological organization to obtain more accurate estimates of the species capacity to face climate change.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article