Insights into the Conformation and Self-Association of a Concentrated Monoclonal Antibody using Isothermal Chemical Denaturation and Nuclear Magnetic Resonance.

Xu, Jianwen; Namanja, Andrew; Chan, Siew Leong; Son, Chelsea; Petros, Andrew M; Sun, Chaohong; Radziejewski, Czeslaw; Ihnat, Peter M

Xu, Jianwen; Namanja, Andrew; Chan, Siew Leong; Son, Chelsea; Petros, Andrew M; Sun, Chaohong; Radziejewski, Czeslaw; Ihnat, Peter M.

Afiliação

Xu J; Drug Product Development Biologic Preformulation, Abbvie Bioresearch Center, Worcester, MA 01605, United States.
Namanja A; Protein & Assay Sciences, Drug Discovery Science & Technology, Abbvie Inc, Waukegan, IL 60031, United States.
Chan SL; Analytical Sciences, Takeda Pharmaceuticals, Westborough, MA 01581, United States; Protein Analytics, Abbvie Bioresearch Center, Worcester, MA 01605, United States.
Son C; Drug Product Development Biologic Preformulation, Abbvie Bioresearch Center, Worcester, MA 01605, United States; Process Analytics, Amgen Inc., Cambridge, MA 02141, United States.
Petros AM; Protein & Assay Sciences, Drug Discovery Science & Technology, Abbvie Inc, Waukegan, IL 60031, United States.
Sun C; Protein & Assay Sciences, Drug Discovery Science & Technology, Abbvie Inc, Waukegan, IL 60031, United States.
Radziejewski C; Protein Analytics, Abbvie Bioresearch Center, Worcester, MA 01605, United States.
Ihnat PM; Drug Product Development Biologic Preformulation, Abbvie Bioresearch Center, Worcester, MA 01605, United States; Protein Biochemistry, Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, United States. Electronic address: peter.ihnat@regeneron.com.

J Pharm Sci ; 110(12): 3819-3828, 2021 12.

Article em En | MEDLINE | ID: mdl-34506864

Assuntos

Anticorpos Monoclonais; Imunoglobulina G; Anticorpos Monoclonais/química; Concentração de Íons de Hidrogênio; Imunoglobulina G/química; Espectroscopia de Ressonância Magnética; Conformação Proteica; Desnaturação Proteica; Viscosidade

Palavras-chave

Aggregation; Conformation; Free energy of unfolding; IgG1; Isothermal chemical denaturation; Monoclonal antibody; NMR; Protein interactions; Self-association; Solution dynamics

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Anticorpos Monoclonais Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Anticorpos Monoclonais Idioma: En Ano de publicação: 2021 Tipo de documento: Article