Functional Analysis of Connexin Channels in Cultured Cells by Neurobiotin Injection and Visualization.

ABSTRACT

Functional gap junction channels between neighboring cells can be assessed by microinjection of low molecular weight tracer substances into cultured cells. The extent of direct intercellular communication can be precisely quantified by this method. This protocol describes the iontophoretic injection and visualisation of Neurobiotin into cultured cells.