Assembly of long L-RNA by native RNA ligation.
Chem Commun (Camb)
; 57(81): 10508-10511, 2021 Oct 12.
Article
em En
| MEDLINE
| ID: mdl-34550128
ABSTRACT
Due to their intrinsic nuclease resistance, L-oligonucleotides are being increasingly utilized in the development of molecular tools and sensors. Yet, it remains challenging to synthesize long L-oligonucleotides, potential limiting future applications. Herein, we report straightforward and versitile approach to assemble long L-RNAs from two or more shorter fragments using T4 RNA ligase 1. We show that this approach is compatible with the assembly of several classes of functional L-RNA, which we highlight by generating a 124 nt L-RNA biosensor that functions in serum.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Ligase (ATP)
/
Proteínas Virais
/
RNA
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article