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Upregulation of hemeoxygenase enzymes HO-1 and HO-2 following ischemia-reperfusion injury in connection with experimental cardiac arrest and cardiopulmonary resuscitation: Neuroprotective effects of methylene blue.
Wiklund, Lars; Sharma, Aruna; Patnaik, Ranjana; Muresanu, Dafin F; Sahib, Seaab; Tian, Z Ryan; Castellani, Rudy J; Nozari, Ala; Lafuente, José Vicente; Sharma, Hari Shanker.
Afiliação
  • Wiklund L; International Experimental Central Nervous System Injury & Repair (IECNSIR), Department of Surgical Sciences, Anesthesiology & Intensive Care Medicine, Uppsala University Hospital, Uppsala University, Uppsala, Sweden.
  • Sharma A; International Experimental Central Nervous System Injury & Repair (IECNSIR), Department of Surgical Sciences, Anesthesiology & Intensive Care Medicine, Uppsala University Hospital, Uppsala University, Uppsala, Sweden. Electronic address: lars.wiklund@surgsci.uu.se.
  • Patnaik R; Department of Biomaterials, School of Biomedical Engineering, Indian Institute of Technology, Banaras Hindu University, Varanasi, India.
  • Muresanu DF; Department of Clinical Neurosciences, University of Medicine & Pharmacy, Cluj-Napoca, Romania; "RoNeuro" Institute for Neurological Research and Diagnostic, Cluj-Napoca, Romania.
  • Sahib S; Department of Chemistry & Biochemistry, University of Arkansas, Fayetteville, AR, United States.
  • Tian ZR; Department of Chemistry & Biochemistry, University of Arkansas, Fayetteville, AR, United States.
  • Castellani RJ; Department of Pathology, University of Maryland, Baltimore, MD, United States.
  • Nozari A; Anesthesiology & Intensive Care, Massachusetts General Hospital, Boston, MA, United States.
  • Lafuente JV; LaNCE, Department of Neuroscience, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.
  • Sharma HS; International Experimental Central Nervous System Injury & Repair (IECNSIR), Department of Surgical Sciences, Anesthesiology & Intensive Care Medicine, Uppsala University Hospital, Uppsala University, Uppsala, Sweden. Electronic address: sharma@surgsci.uu.se.
Prog Brain Res ; 265: 317-375, 2021.
Article em En | MEDLINE | ID: mdl-34560924
ABSTRACT
Oxidative stress plays an important role in neuronal injuries after cardiac arrest. Increased production of carbon monoxide (CO) by the enzyme hemeoxygenase (HO) in the brain is induced by the oxidative stress. HO is present in the CNS in two isoforms, namely the inducible HO-1 and the constitutive HO-2. Elevated levels of serum HO-1 occurs in cardiac arrest patients and upregulation of HO-1 in cardiac arrest is seen in the neurons. However, the role of HO-2 in cardiac arrest is not well known. In this review involvement of HO-1 and HO-2 enzymes in the porcine brain following cardiac arrest and resuscitation is discussed based on our own observations. In addition, neuroprotective role of methylene blue- an antioxidant dye on alterations in HO under in cardiac arrest is also presented. The biochemical findings of HO-1 and HO-2 enzymes using ELISA were further confirmed by immunocytochemical approach to localize selective regional alterations in cardiac arrest. Our observations are the first to show that cardiac arrest followed by successful cardiopulmonary resuscitation results in significant alteration in cerebral concentrations of HO-1 and HO-2 levels indicating a prominent role of CO in brain pathology and methylene blue during CPR followed by induced hypothermia leading to superior neuroprotection after return of spontaneous circulation (ROSC), not reported earlier.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão / Fármacos Neuroprotetores / Parada Cardíaca Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão / Fármacos Neuroprotetores / Parada Cardíaca Idioma: En Ano de publicação: 2021 Tipo de documento: Article