Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes.
Sci Rep
; 11(1): 19033, 2021 09 24.
Article
em En
| MEDLINE
| ID: mdl-34561481
ABSTRACT
Keratinocytes, the main cell type of the skin, are one of the most exposed cells to environmental factors, providing a first defence barrier for the host and actively participating in immune response. In fact, keratinocytes express pattern recognition receptors that interact with pathogen associated molecular patterns and damage associated molecular patterns, leading to the production of cytokines and chemokines, including interleukin (IL)-6. Herein, we investigated whether mechanical energy transported by low intensity ultrasound (US) could generate a mechanical stress able to induce the release of inflammatory cytokine such IL-6 in the human keratinocyte cell line, HaCaT. The extensive clinical application of US in both diagnosis and therapy suggests the need to better understand the related biological effects. Our results point out that US promotes the overexpression and secretion of IL-6, associated with the activation of nuclear factor-κB (NF-κB). Furthermore, we observed a reduced cell viability dependent on exposure parameters together with alterations in membrane permeability, paving the way for further investigating the molecular mechanisms related to US exposure.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
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Queratinócitos
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Interleucina-6
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Mediadores da Inflamação
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Ondas Ultrassônicas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article