Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1.
J Cell Biol
; 220(11)2021 11 01.
Article
em En
| MEDLINE
| ID: mdl-34570198
ABSTRACT
The fate of hematopoietic stem and progenitor cells (HSPCs) is regulated by their interaction with stromal cells in the bone marrow. However, the cellular mechanisms regulating HSPC interaction with these cells and their potential impact on HSPC polarity are still poorly understood. Here we evaluated the impact of cell-cell contacts with osteoblasts or endothelial cells on the polarity of HSPC. We found that an HSPC can form a discrete contact site that leads to the extensive polarization of its cytoskeleton architecture. Notably, the centrosome was located in proximity to the contact site. The capacity of HSPCs to polarize in contact with stromal cells of the bone marrow appeared to be specific, as it was not observed in primary lymphoid or myeloid cells or in HSPCs in contact with skin fibroblasts. The receptors ICAM, VCAM, and SDF1 were identified in the polarizing contact. Only SDF1 was independently capable of inducing the polarization of the centrosome-microtubule network.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Medula Óssea
/
Quimiocina CXCL12
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article