Factors involved in production of helper virus-free retrovirus vectors.
Somat Cell Mol Genet
; 12(2): 175-83, 1986 Mar.
Article
em En
| MEDLINE
| ID: mdl-3457477
ABSTRACT
Retrovirus vectors allow efficient transfer of genetic material into cells. We describe an improved method for making cell lines which secrete broad host range retrovirus vectors in the absence of helper virus. This method was used to make virus-producing cell lines from several retrovirus vector constructions that encode dominant selectable markers. Virus titers from such lines exceeded 10(6) colony-forming units per milliliter of medium exposed to the cells. Cell lines that secreted certain vectors remained free of helper virus, while cell lines made using other vectors always secreted helper virus. Secretion of helper virus apparently depended on recombination between vector and the retrovirus packaging system, and factors involved in this event were investigated.
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Base de dados:
MEDLINE
Assunto principal:
Retroviridae
/
Transformação Celular Viral
/
Vetores Genéticos
/
Vírus Auxiliares
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article