Multicommutated Flow Analysis System for Determination of Horseradish Peroxidase and Its Inhibitors.
Molecules
; 26(18)2021 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-34577101
ABSTRACT
A fully mechanized multicommutated flow analysis (MCFA) system dedicated to determining horseradish peroxidase (HRP) activity was developed. Detection was conducted using a flow-through optoelectronic detector-constructed of paired LEDs operating according to the paired emitter-detector diode (PEDD) principle. The PEDD-MCFA system is dedicated to monitoring the enzyme-catalyzed oxidation of p-phenylenediamine (pPD) by a hydrogen peroxide. Under optimized conditions, the presented bioanalytical system was characterized by a linear response range (33.47-200 U/L) with a detection limit at 10.54 U/L HRP activity and 1.66 mV·L/U sensitivity, relatively high throughput (12 signals recordings per hour), and acceptable precision (RSD below 6%). Additionally, the utility of the developed PEDD-MCFA system for the determination of HRP inhibitors allowing the detection of selected thiols at micromolar levels, is demonstrated. The practical utility of the flow system was illustrated by the analysis of some dietary supplements containing L-cysteine, N-acetylcysteine, and L-glutathione.
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Base de dados:
MEDLINE
Assunto principal:
Análise de Injeção de Fluxo
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Técnicas Analíticas Microfluídicas
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Técnicas Eletroquímicas
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Ensaios Enzimáticos
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Peroxidase do Rábano Silvestre
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Nefelometria e Turbidimetria
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article