Chikungunya viruses containing the A226V mutation detected retrospectively in Cameroon form a new geographical subclade.
Int J Infect Dis
; 113: 65-73, 2021 Dec.
Article
em En
| MEDLINE
| ID: mdl-34592442
ABSTRACT
BACKGROUND:
Chikungunya virus (CHIKV) is a re-emerging arbovirus associated with sporadic outbreaks in Cameroon since 2006. Viral whole genomes were generated to analyze the origins of evolutionary lineages, the potential of emergence/re-emergence, and to infer transmission dynamics of recent Cameroon CHIKV outbreak strains.METHODS:
Samples collected between 2016 and 2019 during CHIKV outbreaks in Cameroon were screened for CHIKV using reverse transcription PCR (RT-PCR), followed by whole genome sequencing of positive samples.RESULTS:
Three coding-complete CHIKV genomes were obtained from samples, which belong to an emerging sub-lineage of the East/Central/South African genotype and formed a monophyletic taxon with previous Central African strains. This clade, which we have named the new Central African clade, appears to be evolving at 3.0 × 10-4 nucleotide substitutions per site per year (95% highest posterior density (HPD) interval of 1.94 × 10-4 to 4.1 × 10-4). Notably, mutations in the envelope proteins (E1-A226V, E2-L210Q, and E2-I211T), which are known to enhance CHIKV adaptability and infectious potential in Aedes albopictus, were present in all strains and mapped to established high-density Ae. albopictus populations.CONCLUSIONS:
These new CHIKV strains constitute a conserved genomic pool of an emerging sub-lineage, reflecting a putative vector host adaptation to Ae. albopictus, which has practically displaced Aedes aegypti from select regions of Cameroon.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Vírus Chikungunya
/
Aedes
/
Febre de Chikungunya
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article