Improvement in image quality via the pseudo confocal effect in multidirectional digital scanned laser light-sheet microscopy.
Opt Express
; 29(15): 24278-24288, 2021 Jul 19.
Article
em En
| MEDLINE
| ID: mdl-34614676
ABSTRACT
Multidirectional digital scanned laser light-sheet microscopy (mDSLM) cannot be used with the current pseudo confocal system to reduce blurring and background signals. The multiline scanning for light-sheet illumination and the simple image construction proposed in this study are alternative to the pseudo confocal system. We investigate the effectiveness of our pseudo confocal method combined with mDSLM on artificial phantoms and biological samples. The results indicate that image quality from mDSLM can be improved by the confocal effect; their combination is effective and can be applied to biological investigations.
Texto completo:
1
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article