Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis.
Nat Cell Biol
; 23(10): 1073-1084, 2021 10.
Article
em En
| MEDLINE
| ID: mdl-34616024
ABSTRACT
Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
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Clatrina
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Integrina beta1
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Proteínas rab de Ligação ao GTP
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Dinaminas
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Peptídeos e Proteínas de Sinalização Intracelular
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Endocitose
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article