Modulations of blood biochemical parameters of golden mahseer, Tor putitora following exposures to single and mixed organophosphate.

Increasing pesticide application is a serious threat to human health and biodiversity. In nature, pesticides prevail in mixtures; therefore the joint effects of pesticides should be taken into consideration due to their priority in toxicity research when aiming at realistic evaluations. In this study, individual and mixture toxicity of the commonly used organophosphate insecticides- chlorpyrifos and dichlorvos was explored. Healthy and clinically active juveniles of golden mahseer (Tor putitora) were exposed to sub-lethal doses (10% of the 96 h-LC50) of the chlorpyrifos, dichlorvos, and their mixture. Blood sampling was conducted after 24 h and 96 h of exposure, followed by a 1 week recovery period. Among the examined biochemical parameters; blood glucose in dichlorvos treatment; alanine aminotransferase and alkaline phosphatase in chlorpyrifos and dichlorvos treatments; and aspartate aminotransferase and urea in mixture pesticide treatments were elevated. In contrast, blood albumin and triglycerides were diminished in mixture pesticide treatments. Vital organs like kidney and liver of the tested animals were compromised to different magnitudes in different pesticide treatments. Kidney was found to be more sensitive than liver in terms of pesticide toxicity during this short exposure experiment. This study revealed that most of the biomarkers were mainly affected at a later exposure phase (after 96 h) and steadily recovered during the depuration period.