Carbon starvation-induced synthesis of GDH2 and PEPCK is essential for the survival of Pichia pastoris.
Biochem Biophys Res Commun
; 581: 25-30, 2021 12 03.
Article
em En
| MEDLINE
| ID: mdl-34653675
ABSTRACT
The industrial yeast Pichia pastoris can utilize amino acids as the sole source of carbon. It possesses a post-transcriptional regulatory circuit that governs the synthesis of cytosolic glutamate dehydrogenase 2 (GDH2) and phosphoenolpyruvate carboxykinase (PEPCK), key enzymes of amino acid catabolism. Here, we demonstrate that the post-transcriptional regulatory circuit is activated during carbon starvation resulting in the translation of GDH2 and PEPCK mRNAs. GDH2 and PEPCK synthesis is abrogated in Δatg1 indicating a key role for autophagy or an autophagy-related process. Finally, carbon-starved Δgdh2 and Δpepck exhibit poor survival. This study demonstrates a key role for amino acid catabolism during carbon starvation, a phenomenon hitherto unreported in other yeast species.
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Proteínas Fúngicas
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Carbono
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Desidrogenase de Glutamato (NADP/)
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Fosfoenolpiruvato Carboxiquinase (ATP)
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Saccharomycetales
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article