Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy.
STAR Protoc
; 2(4): 100863, 2021 12 17.
Article
em En
| MEDLINE
| ID: mdl-34661171
ABSTRACT
We describe sample preparation and visualization of fluorescently tagged cellulose synthases in cellulose synthase complexes at the plasma membrane of Arabidopsis hypocotyl epidermal cells using live-cell imaging via spinning disk microscopy. We present a technique for sample mounting that may be suitable for imaging other samples. Additionally, we offer free, open-source solutions for image analysis and provide extensive troubleshooting suggestions. For complete information on the use and execution of this protocol, please refer to McFarlane et al., 2021.
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Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
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Hipocótilo
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Proteínas de Arabidopsis
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Glucosiltransferases
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Microscopia
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article