SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart.
Life Sci Alliance
; 4(12)2021 12.
Article
em En
| MEDLINE
| ID: mdl-34663679
ABSTRACT
Extracellular vesicles (EVs) mediate intercellular signaling by transferring their cargo to recipient cells, but the functional consequences of signaling are not fully appreciated. RBC-derived EVs are abundant in circulation and have been implicated in regulating immune responses. Here, we use a transgenic mouse model for fluorescence-based mapping of RBC-EV recipient cells to assess the role of this intercellular signaling mechanism in heart disease. Using fluorescent-based mapping, we detected an increase in RBC-EV-targeted cardiomyocytes in a murine model of ischemic heart failure. Single cell nuclear RNA sequencing of the heart revealed a complex landscape of cardiac cells targeted by RBC-EVs, with enrichment of genes implicated in cell proliferation and stress signaling pathways compared with non-targeted cells. Correspondingly, cardiomyocytes targeted by RBC-EVs more frequently express cellular markers of DNA synthesis, suggesting the functional significance of EV-mediated signaling. In conclusion, our mouse model for mapping of EV-recipient cells reveals a complex cellular network of RBC-EV-mediated intercellular communication in ischemic heart failure and suggests a functional role for this mode of intercellular signaling.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Nuclear
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Transdução de Sinais
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Eritrócitos
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Análise de Célula Única
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Vesículas Extracelulares
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RNA-Seq
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Insuficiência Cardíaca
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Infarto do Miocárdio
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Miocárdio
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article