Physiologic biomechanics enhance reproducible contractile development in a stem cell derived cardiac muscle platform.
Nat Commun
; 12(1): 6167, 2021 10 25.
Article
em En
| MEDLINE
| ID: mdl-34697315
ABSTRACT
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) allow investigations in a human cardiac model system, but disorganized mechanics and immaturity of hPSC-CMs on standard two-dimensional surfaces have been hurdles. Here, we developed a platform of micron-scale cardiac muscle bundles to control biomechanics in arrays of thousands of purified, independently contracting cardiac muscle strips on two-dimensional elastomer substrates with far greater throughput than single cell methods. By defining geometry and workload in this reductionist platform, we show that myofibrillar alignment and auxotonic contractions at physiologic workload drive maturation of contractile function, calcium handling, and electrophysiology. Using transcriptomics, reporter hPSC-CMs, and quantitative immunofluorescence, these cardiac muscle bundles can be used to parse orthogonal cues in early development, including contractile force, calcium load, and metabolic signals. Additionally, the resultant organized biomechanics facilitates automated extraction of contractile kinetics from brightfield microscopy imaging, increasing the accessibility, reproducibility, and throughput of pharmacologic testing and cardiomyopathy disease modeling.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Miócitos Cardíacos
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Células-Tronco Pluripotentes
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Coração
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Miocárdio
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article