A heterotrinuclear bioinspired coordination complex capable of binding to DNA and emulation of nuclease activity.
J Inorg Biochem
; 226: 111631, 2022 01.
Article
em En
| MEDLINE
| ID: mdl-34717251
ABSTRACT
The investigation of compounds capable of strongly and selectively interacting with DNA comprises a field of research in constant development. In this work, we demonstrate that a trinuclear coordination complex based on a dinuclear Fe(III)Zn(II) core designed for biomimicry of the hydrolytic enzyme kidney bean purple acid phosphatase, containing an additional pendant arm coordinating a Pd(II) ion, has the ability to interact with DNA and to promote its hydrolytic cleavage. These results were found through analysis of plasmid DNA interaction and cleavage by the trinuclear complex 1 and its derivatives 2 and 3, in addition to the analysis of alteration in the DNA structure in the presence of the complexes through circular dichroism and DNA footprinting techniques. The suggested covalent interaction of the palladium-containing complex with DNA was analysed using an electrophoretic mobility assay, circular dichroism, high resolution gel separation techniques and kinetic analysis. This is a new and promising metal complex targeted to nucleic acids and acting in two separate ways strong DNA interaction and hydrolytic cleavage.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
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Desoxirribonucleases
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Clivagem do DNA
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Complexos de Coordenação
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Metais
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article