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Expression of a ß-glucosidase from Trichoderma reesei in Escherichia coli using a synthetic optimized gene and stability improvements by immobilization using magnetite nano-support.
Vázquez-Ortega, Perla Guadalupe; López-Miranda, Javier; Rojas-Contreras, Juan Antonio; Ilina, Anna; Soto-Cruz, Nicolás Oscar; Páez-Lerma, Jesús Bernardo.
Afiliação
  • Vázquez-Ortega PG; Tecnológico Nacional de México/Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote. Col. Nueva Vizcaya, CP 34080, Durango, Dgo., Mexico.
  • López-Miranda J; Tecnológico Nacional de México/Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote. Col. Nueva Vizcaya, CP 34080, Durango, Dgo., Mexico. Electronic address: jlopez@itdurango.edu.mx.
  • Rojas-Contreras JA; Tecnológico Nacional de México/Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote. Col. Nueva Vizcaya, CP 34080, Durango, Dgo., Mexico.
  • Ilina A; Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Blvd. V. Carranza e Ing. José Cárdenas V. Col. República Oriente, CP 25000, Saltillo, Coahuila, Mexico.
  • Soto-Cruz NO; Tecnológico Nacional de México/Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote. Col. Nueva Vizcaya, CP 34080, Durango, Dgo., Mexico.
  • Páez-Lerma JB; Tecnológico Nacional de México/Instituto Tecnológico de Durango, Blvd. Felipe Pescador 1830 Ote. Col. Nueva Vizcaya, CP 34080, Durango, Dgo., Mexico.
Protein Expr Purif ; 190: 106009, 2022 02.
Article em En | MEDLINE | ID: mdl-34742914
The enzymatic conversion of lignocellulosic biomass to fermentable sugars is determined by the enzymatic activity of cellulases; consequently, improving enzymatic activity has attracted great interest in the scientific community. Cocktails of commercial cellulase often have low ß-glucosidase content, leading to the accumulation of cellobiose. This accumulation inhibits the activity of the cellulolytic complex and can be used to determine the enzymatic efficiency of commercial cellulase cocktails. Here, a novel codon optimized ß-glucosidase gene (B-glusy) from Trichoderma reesei QM6a was cloned and expressed in three strains of Escherichia coli (E. coli). The synthetic sequence containing an open reading frame (ORF) of 1491 bp was used to encode a polypeptide of 497 amino acid residues. The ß-glucosidase recombinant protein that was expressed (57 kDa of molecular weight) was purified by Ni agarose affinity chromatography and visualized by SDS-PAGE. The recombinant protein was better expressed in E. coli BL21 (DE3), and its enzymatic activity was higher at neutral pH and 30 °C (22.4 U/mg). Subsequently, the ß-glucosidase was immobilized using magnetite nano-support, after which it maintained >65% of its enzymatic activity from pH 6 to 10, and was more stable than the free enzyme above 40 °C. The maximum immobilization yield had enzyme activity of 97.2%. In conclusion, ß-glucosidase is efficiently expressed in the microbial strain E. coli BL21 (DE3) grown in a simplified culture medium.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Fúngicas / Expressão Gênica / Beta-Glucosidase / Enzimas Imobilizadas / Escherichia coli / Nanopartículas de Magnetita / Hypocreales Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Fúngicas / Expressão Gênica / Beta-Glucosidase / Enzimas Imobilizadas / Escherichia coli / Nanopartículas de Magnetita / Hypocreales Idioma: En Ano de publicação: 2022 Tipo de documento: Article