Regulation of translation by site-specific ribosomal RNA methylation.
Nat Struct Mol Biol
; 28(11): 889-899, 2021 11.
Article
em En
| MEDLINE
| ID: mdl-34759377
ABSTRACT
Ribosomes are complex ribozymes that interpret genetic information by translating messenger RNA (mRNA) into proteins. Natural variation in ribosome composition has been documented in several organisms and can arise from several different sources. A key question is whether specific control over ribosome heterogeneity represents a mechanism by which translation can be regulated. We used RiboMeth-seq to demonstrate that differential 2'-O-methylation of ribosomal RNA (rRNA) represents a considerable source of ribosome heterogeneity in human cells, and that modification levels at distinct sites can change dynamically in response to upstream signaling pathways, such as MYC oncogene expression. Ablation of one prominent methylation resulted in altered translation of select mRNAs and corresponding changes in cellular phenotypes. Thus, differential rRNA 2'-O-methylation can give rise to ribosomes with specialized function. This suggests a broader mechanism where the specific regulation of rRNA modification patterns fine tunes translation.
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Base de dados:
MEDLINE
Assunto principal:
Ribossomos
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Biossíntese de Proteínas
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RNA Ribossômico
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Processamento Pós-Transcricional do RNA
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Proteínas Proto-Oncogênicas c-myc
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article