miR-141-5p Affects the Cell Proliferation and Apoptosis by Targeting BTG1 in Cervical Cancer.
Cancer Biother Radiopharm
; 2021 Nov 12.
Article
em En
| MEDLINE
| ID: mdl-34767738
ABSTRACT
Background:
MicroRNAs have been discovered to have the possibility to play a significant role in cancer development. It has been found that miR-141-5p is upregulated in various cancers. However, the functions of miR-141-5p in cervical cancer have rarely been reported.Methods:
The expression level of miR-141-5p was assessed in cervical cancer tissues and cell lines by RT-qPCR. The function of miR-141-5p in C33A and HeLa cells was detected by CCK-8, and colony formation, wound-healing, transwell chamber, and flow cytometry assays. Dual luciferase reporter was carried out to identify the interaction between miR-141-5p and BTG antiproliferation factor 1 (BTG1).Results:
miR-141-5p was upregulated in cervical cancer and was negatively associated with the prognosis of patients with cervical cancer. Functional analyses demonstrated that silenced miR-141-5p expression inhibited the cell proliferation, migration, and invasion, and alleviated apoptosis of C33A and HeLa cells. In addition, miR-141-5p suppresses the activity of BTG1-3'-UTR. Rescue assays demonstrated that the cervical cancer progression is suppressed by miR-141-5p inhibitor and retrieved by sh-BTG1.Conclusions:
The authors' findings reveal that miR-141-5p exerts its role through targeting BTG1 in cervical cancer progression, indicating that miR-141-5p may represent a promising target for the treatment of cervical cancer patients. The Clinical Trial Registration number (2019-KY013).
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Base de dados:
MEDLINE
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En
Ano de publicação:
2021
Tipo de documento:
Article