Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host.
Biol Open
; 10(11)2021 11 15.
Article
em En
| MEDLINE
| ID: mdl-34787304
ABSTRACT
The protein composition of the cestode Schistocephalus solidus was measured in an experiment simulating the trophic transmission of the parasite from a cold-blooded to a warm-blooded host. The first hour of host colonisation was studied in a model experiment, in which sticklebacks Gasterosteus aculeatus infected with S. solidus were heated at 40°C for 1â
h. As a result, a decrease in the content of one tegument protein was detected in the plerocercoids of S. solidus. Sexual maturation of the parasites was initiated in an experiment where S. solidus larvae were taken from fish and cultured in vitro at 40°C for 48â
h. Temperature-independent changes in the parasite proteome were investigated by incubating plerocercoids at 22°C for 48â
h in culture medium. Analysis of the proteome allowed us to distinguish the temperature-induced genes of S. solidus, as well as to specify the molecular markers of the plerocercoid and adult worms. The main conclusion of the study is that the key enzymes of long-term metabolic changes (glycogen consumption, protein production, etc.) in parasites during colonisation of a warm-blooded host are induced by temperature.
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MEDLINE
Assunto principal:
Proteoma
/
Smegmamorpha
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Doenças dos Peixes
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Temperatura Alta
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Interações Hospedeiro-Parasita
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article