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Single-cell transcriptomic characterization of a gastrulating human embryo.
Tyser, Richard C V; Mahammadov, Elmir; Nakanoh, Shota; Vallier, Ludovic; Scialdone, Antonio; Srinivas, Shankar.
Afiliação
  • Tyser RCV; Department of Physiology, Anatomy and Genetics, South Parks Road, University of Oxford, Oxford, UK.
  • Mahammadov E; Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München-German Research Center for Environmental Health, Munich, Germany.
  • Nakanoh S; Institute of Functional Epigenetics, Helmholtz Zentrum München-German Research Center for Environmental Health, Neuherberg, Germany.
  • Vallier L; Institute of Computational Biology, Helmholtz Zentrum München-German Research Center for Environmental Health, Neuherberg, Germany.
  • Scialdone A; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge, UK.
  • Srinivas S; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge, UK.
Nature ; 600(7888): 285-289, 2021 12.
Article em En | MEDLINE | ID: mdl-34789876
ABSTRACT
Gastrulation is the fundamental process in all multicellular animals through which the basic body plan is first laid down1-4. It is pivotal in generating cellular diversity coordinated with spatial patterning. In humans, gastrulation occurs in the third week after fertilization. Our understanding of this process in humans is relatively limited and based primarily on historical specimens5-8, experimental models9-12 or, more recently, in vitro cultured samples13-16. Here we characterize in a spatially resolved manner the single-cell transcriptional profile of an entire gastrulating human embryo, staged to be between 16 and 19 days after fertilization. We use these data to analyse the cell types present and to make comparisons with other model systems. In addition to pluripotent epiblast, we identified primordial germ cells, red blood cells and various mesodermal and endodermal cell types. This dataset offers a unique glimpse into a central but inaccessible stage of our development. This characterization provides new context for interpreting experiments in other model systems and represents a valuable resource for guiding directed differentiation of human cells in vitro.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perfilação da Expressão Gênica / Embrião de Mamíferos / Gastrulação / Análise de Célula Única / Transcriptoma / Gástrula Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perfilação da Expressão Gênica / Embrião de Mamíferos / Gastrulação / Análise de Célula Única / Transcriptoma / Gástrula Idioma: En Ano de publicação: 2021 Tipo de documento: Article