Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons.

Bessa-Neto, Diogo; Beliu, Gerti; Kuhlemann, Alexander; Pecoraro, Valeria; Doose, Sören; Retailleau, Natacha; Chevrier, Nicolas; Perrais, David; Sauer, Markus; Choquet, Daniel

Bessa-Neto, Diogo; Beliu, Gerti; Kuhlemann, Alexander; Pecoraro, Valeria; Doose, Sören; Retailleau, Natacha; Chevrier, Nicolas; Perrais, David; Sauer, Markus; Choquet, Daniel.

Afiliação

Bessa-Neto D; University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, F-33000, Bordeaux, France.
Beliu G; Department of Biotechnology and Biophysics, University of Würzburg, Biocenter, Am Hubland, 97074, Würzburg, Germany.
Kuhlemann A; Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Wuerzburg, Wuerzburg, Germany.
Pecoraro V; Department of Biotechnology and Biophysics, University of Würzburg, Biocenter, Am Hubland, 97074, Würzburg, Germany.
Doose S; University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, F-33000, Bordeaux, France.
Retailleau N; Department of Biotechnology and Biophysics, University of Würzburg, Biocenter, Am Hubland, 97074, Würzburg, Germany.
Chevrier N; University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, F-33000, Bordeaux, France.
Perrais D; University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, F-33000, Bordeaux, France.
Sauer M; University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, F-33000, Bordeaux, France.
Choquet D; Department of Biotechnology and Biophysics, University of Würzburg, Biocenter, Am Hubland, 97074, Würzburg, Germany. m.sauer@uni-wuerzburg.de.

Nat Commun ; 12(1): 6715, 2021 11 18.

Article em En | MEDLINE | ID: mdl-34795271

Assuntos

Aminoácidos/metabolismo; Epitopos/metabolismo; Proteínas de Membrana/metabolismo; Neurônios/metabolismo; Coloração e Rotulagem/métodos; Animais; Células COS; Chlorocebus aethiops; Feminino; Células HEK293; Humanos; Masculino; Camundongos Endogâmicos C57BL; Microscopia Confocal/métodos; Imagem Óptica/métodos; Ratos Sprague-Dawley; Receptores de AMPA/metabolismo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / Aminoácidos / Proteínas de Membrana / Epitopos / Neurônios Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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